The title of Earl of Cambrige was created several times in the Peerage of England, and since 1362 the title has been closely associated with the Royal Family (see also Duke of Cambridge, Marquess of Cambridge). The first Earl of the fourth creation, the Marquess of Hamilton, was at the time sixth in line to the Crown of Scotland (after the Duke of Rothesy, later King Charles I, his sister Elizabeth and her children); his grandfather Lord Arran had been heir-presumptive to, and Regent for, Mary Queen of Scots.